Key takeaways
- HPLC quantifies how much of a sample is the target compound.
- A clean result: one sharp dominant peak over a flat baseline.
- ≥99% means the target is at least 99% of the measured area.
- HPLC proves purity; LC-MS proves identity — a release needs both.
What HPLC measures
High-performance liquid chromatography (HPLC) separates the components of a sample so their relative amounts can be measured. For a reference peptide, it quantifies how much of the sample is the target compound versus impurities.
Reading the chromatogram
A clean result shows one dominant, sharp peak — the target compound — over a flat baseline. The tall peak is the peptide; the flat baseline is the absence of impurities. A ≥99% result means the target accounts for at least 99% of the measured area.
Purity vs identity
HPLC confirms purity — how much target is present — but not identity. Identity (that the molecule is what the label says) is confirmed separately by LC-MS. A complete release requires both.
Frequently asked
Research use only. This reference material is for in-vitro laboratory research. Not for human or animal consumption.
